Gatekeepers of recombination
Article Abstract:
The Rad52 protein may act as 'gatekeeper' in homologous recombination. It has been established that human Rad52 selectively binds to DNA ends, protecting them from exonuclease attack. It is possible that Rad52 assists in loading Rad51 onto a single-stranded DNA end. This research has also produced strong evidence that purified Rad52 strongly promotes end-to-end joining of DNA, both between and within molecules. The researchers suggest that Rad52 and Ku are competing agents, channelling the ends of a double-strand break into alternative repair pathways.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1999
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Capture of retrotransposon DNA at the sites of chromosomal double-strand breaks
Article Abstract:
The mechanics of chromosomal repair in Saccharomyces cerevisiae was studied by expressing site-specific HO endonuclease that cuts the mating-type locus. Results showed that non-homologous end-joinings perform repair double-strand breaks in the absence of homologous recombination. The insertion of new base pairs came from the retrotransposon Ty-1 element. The process might explain why pseudogenes and long and short interspersed sequences have been inserted at various locations in the mammalian genome.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1996
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Silencing factors participate in DNA repair and recombination in Saccharomyces cerevisiae
Article Abstract:
Researchers have investigated the role of Sir4, which is involved in transcriptional silencing at telomeres and HM loci, in illegitimate recombination. This involved using the yeast two-hybrid assay to indicate that Hdf1, a yeast homologue of Ku protein, interacts with Sir4. It is possible that Hdf1 functions by first recruiting Sir4, then Sir2 and Sir3, which interact with Sir4, to the DNA ends created by the DNA double-strand break.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1997
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