Technical note: the use of a compartmental model to estimate the de novo production rate of N-methylhistidine in cattle

Article Abstract:

An alternative method to quantify muscle protein breakdown in cattle was developed. The method is based on the measurement of the de novo synthesis of the metabolite N-methylhistidine (NMH) using a compartmental model of the plasma kinetics of the stable NMH isotope, methyl-2H3-N-methylhistidine. NMH estimates derived from the method correlated well with estimates from urinary NMH excretion, indicating that the compartmental model could be an alternative method in estimations of NMH production as an assessment of muscle protein breakdown in cattle.

Measurement, Muscle proteins, Protein metabolism

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Technical note: preservation of tissues and gastrointestinal tract portions by plastic coating or plastination

Article Abstract:

Plastic coating and plastination,two methods of preserving gastrointestinal tract organs and tissues were investigated and compared. Specimens used were immediately removed within 2 hours after the animals' death and were prepared accordingly. The plastic coating method was found to be more simple and inexpensive, while, plastinated specimens were more flexible, durable, and lifelike, in addition to preserving more anatomical structures. Both techniques proved to be valuable tools for postmortem studies of organs and tissues.

Usage, Plastics, Protection and preservation, Gastrointestinal system, Silicones

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Technical note: a noninvasive procedure for measuring goat heart rates

Article Abstract:

A noninvasive procedure for obtaining goat heart rates was developed by comparing the results obtained from FM radio transmitters and heart rate monitors attached to unanesthesized and unrestrained mixed breed goats. Results of the study show that radio transmitters can be utilized to accurately measure heart rates from goats basing on its near approximate results to heart rate monitors.

Research, Heart beat, Heart rate, Goats, Radios, Telemetry, Radio receivers

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