The 1.7 A crystal structure of the regulator of chromosome condensation (RCC1) reveals a seven-bladed propeller

Article Abstract:

The gene which encodes the regulator of chromosome condensation (RCC1) was cloned because it can complement the phenotype of the Hamster cell line tsBN2. This experiences premature chromosome condensation in the G1 phase of the cycle. RCC1 is probably an important part of controlling nucleo-cytoplasmic transport. It is also thought to be able to bind to DNA. X-ray crystallography has been used to study human RCC1, which is shaped like a seven-bladed propeller, and is made up of internal repeats of 51-68 residues on each blade. This explains how a range of mutations occur.

Research and Development in the Physical, Engineering, and Life Sciences, Research and Testing Services, Scientific Research and Development Services, Genetic Engineering, Research & Development-Universities, Analysis, Cloning, Genetic research, University research, Premature chromosome condensation

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Crystal structure of the GTPase-activating domain of human p120GAP and implications for the interaction with Ras

Article Abstract:

X-ray crystallographic analysis of the catalytically active 334 C-terminal residues, which is the GTPase-activating domain of human p120GAP, gives a molecular structure at 3 angstrom resolution. The GTPase activating proteins (GAP) are involved in the ras mediated regulation. The structure shows that two arginines at 789 and 903 are crucial for the interaction between GAP and ras protein. The Arg 903 interacts with the main chain containing the Arg789. This indicates that the Arg 789 is involved in the catalysis, and it stabilizes the orientation.

Usage, Carrier proteins, Transport proteins, G proteins, X-ray crystallography

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Bioorganic synthesis of lipid-modified proteins for the study of signal transduction

Article Abstract:

It is possible to chemically modify Ras proteins with lipopeptides to allow these hybrid proteins to insert into artificial and biological membranes. The neo-lipoproteins have been shown to be efficient tools for biophysical, biochemical and biological experiments. This research used a strategy involving the bacterial expression of a carboxy-terminally truncated non-lipidated protein, the chemical synthesis of differently lipidated peptides representing the C terminus of the proteins, and their covalent coupling.

Cellular signal transduction, Lipid metabolism

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