The very-long-chain acyl-CoA dehydrogenase gene maps to pig chromosome 12

Article Abstract:

The very-long-chain acyl-CoA dehydrogenase (ACADVL) was subjected to polymerase chain reaction amplification. Primers for the gene were prepared from a bovine cDNA sequence aligned with the human ACADVL gene. ACADVL is an enzyme that acts as a catalyst in the beta-oxidation of long-chain fatty acids. Thus, a defective ACADVL gene causes deficiency of the beta-oxidation process, which, in turn, is associated with cardiomyopathy, skeletal myopathy, liver dysfunction and sudden death in childhood.

Fatty acid desaturases

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Rapid communication: cloning of a pig full-length natural resistance associated macrophage protein (NRAMP1) cDNA

Article Abstract:

Degenerate PCR primers were used to amplify a region of pig NRAMP from spleen cDNA. These primers were then utilized to isolate two different cDNA clones. It was determined that the derived pig protein sequence was similar to NRAMP1 found in humans, cattle and mice. Results indicate an important role for the pig NRMAP1 sequence in natural resistance to infection with intracellular pathogens and in preimmune macrophage function.

Cloning, Natural immunity

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Rapid communication: mapping the pig VCAM1 locus to chromosome 4 using a double-stranded conformation polymorphism marker (VCAM1-2)

Article Abstract:

The three-inch untranslated region of ovine VCAM1 was sequenced using PCR-based genotyping. The analysis resulted in the identification of three VCAM1 alleles based on double strand conformation polymorphisms and heteroduplex analysis of PCR products. The inheritance pattern conforms with the expected Mendelian pattern of segregation. This experiment involved 53 unrelated pigs covering five different breeds.

Animal genetics

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