Visualizing DNA replication in a catalytically active Bacillus, DNA polymerase crystal

Article Abstract:

DNA polymerases copy DNA templates with a high fidelity. The mechanism by which nucleotides are correctly incorporated is not known. High-resolution crystal structures of a Bacillus stearothemophilus DNA polymerase I large fragment with DNA primer templates productively bound at the polymerase active site, are presented. The active site remains catalytically active an the polymerase maintains its discriminatory powers between correct and incorrectly paired nucleotides.

Observations, Chromosome replication

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Replication by human DNA polymerase-Iota occur by Hoogsteen base-pairing

Article Abstract:

The crystal structure of hPol(iota) bound to a template primer and an incoming nucleotide is reported. Hoogsteen base-pairing offers a basis for the varied efficiencies and fidelities of hPol(iota) opposite different template bases and it provides an elegant mechanism for promoting replication through minor-groove purine adducts that interfere with replication.

Science & research, Purines

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Eukaryotic polymerases iota and zeta act sequentially to bypass DNA lesions

Article Abstract:

The related human gene hRAD30B is found to encode DNA polymerase Pol iota.

Human genetics

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