Autoscreening of restriction endonucleases for PCR-restriction fragment length polymorphism identification of fungal species, with Pleurotus spp. as an example

Article Abstract:

A molecular method is developed based on PCR-restriction fragment length polymorphism (RFLP) analysis of internal transcribed spacer (ITS) ribosomal DNA sequences in order identify fungal species, with members of the genus Pleurotus as an example. A large number of ITS sequences from this genus are accumulated and a molecular identification method using the PCR-RFLP method is developed for distinguishing Pleurotus species through the DNA sequence analyses.

Usage, Observations, Nucleotide sequence, Base sequence, Polymerase chain reaction

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Tvbgn3, a [beta]-16-glucanase from the biocontrol fungus Trichoderma virens, is involved in Mycoparasitism and control of Pythium ultimum

Article Abstract:

The role of Tvbgn3, a [beta]-1,6-glucanase from Trichoderma virens, is examined by comparison of wild-type (WT) and transformant strains in which Tvbgn3 is disrupted (GKO) or constitutively overexpressed (GOE). The results have shown the involvement of [beta]-1,6-glucanase in mycoparasitism and its relevance in the biocontrol activity of Trichoderma virens.

Bacterial cell walls, Glucans, Structure, Report

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Beringian paleoecology inferred from permafrost-preserved fungal DNA

Article Abstract:

The purpose of the study was to monitor and characterize the fungal composition of ancient permafrost. It was revealed that ancient fungal communities included a diversity of cold-adapted yeasts, dark-pigmented fungi, plant-parasitic fungi, and lichen mycobionts.

Environmental aspects, Frozen ground, Permafrost, Paleoecology

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