Comparison of biological effect of the two different enterotoxin complexes isolated from three different strains of Bacillus cereus

Article Abstract:

A comparative research on two different enterotoxin complexes of Bacillus cereus has been conducted. The samples were subjected to strain levels F837-76, 1230-88 and 0075-95. Non-haemolytic enterotoxin (NHE) protein components were isolated from these strains, while proteins of haemolysin BL (HBL) were segregated from only F837-76 and 1230-88 strains. Findings showed that F837-76 was a highly toxic strain, capable of producing both NHE and HBL. Furthermore, the only disparity between NHE and HBL's level of cytotoxicity is the higher amount of 105 kDA protein of NHE.

Cell-mediated cytotoxicity, Cell mediated cytotoxicity, Hemolysis and hemolysins, Hemolysis

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The bceT gene of Bacillus cereus encodes an enterotoxic protein

Article Abstract:

A study of a toxin gene (bceT) on a 2.9 kb DNA fragment of Bacillus cereus B-4ac shows it to be one of the proteins that causes diarrhea associated with food-borne illnesses. The bceT gene's DNA fragment is able to encode a 336-amino acid polypeptide with a molecular mass of 41039 Da. The gene was cloned and expressed in Escherichia coli, and its nucleotide sequence was determined. The cloned gene exhibited cytotoxic activity and other qualities that confirmed its association with diarrhea caused by food poisoning.

Food poisoning

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The Clostridium perfringens enterotoxin gene is on a transposable element in type A human food poisoning strains

Article Abstract:

The location of the Clostridium perfringens enterotoxin (cpe) gene was investigated. Observations that the gene is unstable and could be gained or lost led to the assumption that it is contained on a mobile element. Polymerase chain reaction results confirmed that the cpe gene is present in the 6.3 kb transposon of type A human food poisoning strains, in addition IS 1470, IS1469 and 1 kb stretches.

Usage, Clostridium, Polymerase chain reaction, Transposons

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