Fungal metabolism of acenaphthene by Cunninghamella elegans
Article Abstract:
Acenaphthene, a polycyclic aromatic hydrocarbon produced by the combustion of fossil fuels and tobacco smoke, can induce nuclear and cytological changes in plants and microorganisms. The metabolic pathway of acenaphtene degradation by Cunninghamella elegans was elucidated. The results showed that acenaphthene is degraded via hydroxylation of both the aliphatic and aromatic rings to hydroxylated and keto derivatives. It is proposed that key site of enzymatic attack resides at the C-1 and C-2 bridge. Comparisons with rat liver microsomes showed that the fungal and mammalian pathways of acenaphthene degradation were similar.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
User Contributions:
Comment about this article or add new information about this topic:
Identification of metabolites from the degradation of fluoranthene by Mycobacterium sp. strain PYR-1
Article Abstract:
The utilization of Mycobacterium sp. strain for the identificatin of metabolites and its capability of degrading fluoranthene were studied. Thin layer and high performance liquid chromatography were used to isolate the meatbolites from the ethyl acetate extracts. These metabolites were identified by UV spectrophotometry, thin-layer chromatography, gas chromatography-mass spectrometry and nuclear magnetic resonance spectra. The utility of Mycobacterium for bioremediation of polycylic aromatic carbons was demonstrated.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
User Contributions:
Comment about this article or add new information about this topic:
Biotransformation of chlorpromazine and methdilazine by Cunninghamella elegans
Article Abstract:
A study was conducted on the in vitro metabolism of N-substituted phenothiazines by Cunninghamella elegans (C. elegans). The drugs chlorpromazine (CPZ) and methdilazine (MDZ) were used as structural probes. Metabolites were extracted by chloroform, separated by high-performance liquid chromatography and characterized by proton nuclear magnetic resonance, mass and ultraviolet spectroscopic analyses. Data indicated that the sulfoxidation and hydroxylation of MDZ and CPZ by C. elegans are catalyzed by cyctochrome P-450.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Functional analysis of the O antigen glucosylation gene cluster of Shigella flexneri bacteriophage SfX. Type IV O antigen modification genes in the genome of shigella flexneri NCTC 8296
- Abstracts: A general model for nucleic acid helicases and their "coupling" within macromolecular machines. Opening of the clamp: an intimate view of an ATP-driven biological machine
- Abstracts: Evidence for an NIH shift in oxidation of naphthalene by the marine cyanobacterium Oscillatoria sp. strain JCM
- Abstracts: The meiotic behavior of an inversion in Caenorhabditis elegans. Two types of sites required for meiotic chromosome pairing in Caenorhabditis elegans
- Abstracts: Physiological properties of a Pseudomonas strain which grows with p-xylene in a two-phase (organic-aqueous) medium