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Biological sciences

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Identification of lipopolysaccharide O antigen synthesis genes required for attachment of the S-layer of Caulobacter crescentus

Article Abstract:

Identification of lipopolysaccharide O antigen synthesis genes required for attachment of the two-dimensional crystalline protein lattice layer, the outer layer or S layer, of Caulobacter crescentus is discussed. The nature of the interaction between the RsaA S-layer subunit protein and the O antigen has been unknown, but findings of a study of the chemical composition of the oligosaccharide will contribute to defining that interaction. The nature of the genes suggests that the C. crescentus O antigen is made up of the 4,6-dideoxyaminohexose perosamine. Glycosyltranferases identified appear to be involved in polymerization of the O antigen.

Canada, Genetic aspects, Identification and classification, Endotoxins, Bacteria, Pathogenic, Pathogenic bacteria, Bacterial cell walls, Membrane fusion, Immune recognition

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S-layer-mediated display of the immunoglobulin G-binding domain of streptococcal protein G on the surface of Caulobacter crescentus: Development of an immunoactive reagent

Article Abstract:

The possibility of constructing functional immunoreactive reagents using the caulobacter S-layer is investigated and it is suggested that in applications where it is necessary to immobilize and isolate immunoglobulin G, S-layer fusion proteins can be used.

Science & research, Research, Usage, Protein binding, Immunoglobulin G, Structure, Western immunoblotting, Western blot

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Polyhydroxybutyrate biosynthesis in Caulobacter crescentus: molecular characterization of the polyhydroxybutyrate synthase

Article Abstract:

Research has been conducted on the Caulobacter crescentus. The ability of C. crescentus to produce polyhydroxybutyrate in the presence of the excess carbon source has been investigated and the results are reported.

Germany, Analysis, Bacteria, Carbon, Biosynthesis, Microbiological research, Polyhydroxybutyrate

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