Improved most-probable-number method to detect sulfate-reducing bacteria with natural media and a radiotracer

Article Abstract:

An innovation on the most-probable-number (MPN) method of detecting sulfate-reducing bacteria (SRB) is presented. The new method utilizes natural media consisting of sterilized sludge or sediment slurries prepared anaerobically. The densities of SRB from a sediment sample in Denmark was examined using the usual MPN method and the new tracer MPN technique using natural media. The new method provided a better estimate of SRB density in the sample.

Identification and classification, Sulfur bacteria

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Improved method for detection of Vibrio parahaemolyticus in seafood

Article Abstract:

Researchers have developed a new medium for isolating Vibrio parahaemolyticus in seafood. It is a chromogenic agar medium containing substrates for beta-galactosidase that turns purple in the presence of V. parahaemolyticus. This medium is superior to thiosulfate citrate bile salts sucrose agar medium, which is usually used to isolate V. parahaemolyticus.

Measurement, Seafood, Vibrio

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Detection of Salmonella enterica in naturally contaminated liquid eggs by loop-mediated isothermal amplification, and characterization of Salmonella isolates

Article Abstract:

Liquid eggs naturally contaminated with low numbers of Salmonella are tested using the loop-mediated isothermal amplification (LAMP) assay. The LAMP assay is rapid, specific, and sensitive for Salmonella detection in liquid eggs and is able to monitor Salmonella contamination in egg-handling plants more reliably.

Science & research, Research, Observations, Salmonella, Eggs (Biology), Egg (Biology)

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