Modified quantitative Association of Official Analytical Chemists sporicidal test for liquid chemical germicides
Article Abstract:
A modified version of the Association of Official Analytical Chemists sporicidal test (966.04) measured the minimum number of spores of Bacillus subtilis or Clostridium sporogenes that have been dried into porcelain penicylinders to be 2.0 x 10 raised to the fifth power. The prescribed sporicidal test 966.04 is used to classify a chemical as a disinfectant but produces inaccurate results when used. The modified version has been evaluated using three commercial disinfectants against three separate groups of spore-labeled cylinders.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Population dynamics of phenol-degrading bacteria in activated sludge determined by gyrB-targeted quantitative PCR
Article Abstract:
A novel approach for quantifying bacterial populations introduced into an activated-sludge microbial community is presented. The approach begins with DNA extraction from the activated sludge, PCR amplification of a gyrB gene fragment and the quantification of the electrophoresed PCR product. Results indicate that nutrient availability was the only factor affecting the experiment, suggesting that the phenol concentration in the aeration tank influenced the alternations in growth tendencies of the microbial populations.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Quantitative PCR method for diagnosis of citrus bacterial canker
Article Abstract:
Researchers describe using an internal standard for the PCR diagnosis of citrus bacterial canker. PCR is a useful tool for diagnosing plant diseases, but the presence of other substances in the plant can inhibit nucleic acid amplification.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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