Sizing chromosomes and megaplasmids in haloarchaea

Article Abstract:

Chromosomal size is conserved in the genera of haloarchaea while plasmid size and composition vary among different strains from the same species and also in the same strain. Pulsed-field gel electrophoresis of different species and strains of haloarchaeal genera indicates the presence of one chromosome between 1.8 to 3 Mb in all members. At least one, but generally many plasmids between 90 and 680 kb are present in supercoiled or linear forms in the haloarchaea.

Usage, Plasmids, Gel electrophoresis, Chromosomes

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Complementation of the hha mutation in Escherichia coli by the ymoA gene from Yersinia enterocolitica: dependence on the gene dosage

Article Abstract:

The ymoA gene from Yersinia enterolitica complements the hha mutation in Escherichia coli when cloned in a medium-copy-number plasmid (pBR322) rather than when cloned in pLG338-30, a low-copy-number plasmid. This may be a result of higher dosage of the hha gene present in the former plasmid. The inefficiency in complementing the hha allele is due to structural differences between Hha and YmoA.

Microbial mutation

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Association between intimin (eae) and EspB gene subtypes in attaching and effacing Escherichia coli strains isolated from diarrhoeic lambs and goat kids

Article Abstract:

Research describes the variability of the locus for enterocyte effacement genes eae and espB, encoding intimin and EspB, respectively, and their interaction with their host attaching and effacing Escherichia coli isolates from diarrhoeic lambs and goats. Results show that most strains exhibit beta-intimin and espB genes identical to rabbit E.coli.

Spain, Statistical Data Included, Analysis, Bacterial adhesion, Diarrhea, Genetic research, Medical bacteriology

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