Small, acid-soluble spore proteins of the alpha/beta type do not protect the DNA in Bacillus subtilis spores against base alkylation

Article Abstract:

The study examined how alpha/beta-type small, acid-soluble proteins (SASP) contribute to the protection of DNA in Bacillus subtilis spores against alkylation by ethyl methanesulfonate (EMS). EMS treatment resulted in the death of both wild-type spores and spores lacking alpha/beta-type SASP. Results indicated that DNA damage was partly how EMS was able to kill dormant spores. It was concluded that alpha/beta-type SASP does not play a role in protecting spore DNA from EMS-related damage.

Alkylation, Alkylation (Petroleum refining)

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Small, acid-soluble proteins bound to DNA protect Bacillus subtilis spores from killing by dry heat

Article Abstract:

Dry bacillus subtilis spores deficient in the two primary small, acid-soluble protein (SASP) alpha and beta DNA-binding proteins are more susceptible to dry heat than wild-type spores indicating that SASP alpha and beta protect the spore DNA against the harmful effects of dry heat. The DNA from the heated spores contain more single-strand break whereas, mutant and wild-type survivors of dry heat show high frequency of mutations.

Protection and preservation

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Heat killing of Bacillus subtilis spores in water is not due to oxidative damage

Article Abstract:

A study was conducted to analyze factors influencing the heat killing of Bacillus subtilis spores in water. Heat-killed spores were examined for oxidative damage to both proteins and DNA. The spores were prepared and cleaned before being suspended in water in capped tubes. Experimental results indicated that oxidative damage to spore macromolecules do not influence spore killing by heat.

DNA, Water, Water analysis

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