Development and testing of a bacterial biosensor for toluene-based environmental contaminants
Article Abstract:
A bacterial biosensor for benzene, toluene and similar compounds appears to be capable of accurately measuring environmental contaminants, making it a potentially inexpensive tool for field-ready assays. A reporter plasmid was constructed by placing the luc gene for firefyl luciferase directly under the control of the P(sub u) promoter and the xylR transcriptional activator of Pseudomonas putida mt-2. The biosensor cells were shown to detect benzene, toluene and xylene contamination in soil and water samples with high sensitivity and quantitative accuracy.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Community analysis of biofilters using fluorescence in situ hybridization including a new probe for the Xanthomonas branch of the class Proteobacteria
Article Abstract:
A 16S rRNA-targeted oligonucleotide probe is described that can hybridize with the Xanthomonas branch of the Proteobacteria. These bacteria are used as biofilters to filter waste gases from air during industrial processes.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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