Three phylogenetic groups of nodA and nifH genes in Sinorhizobium and Mesorhizobium isolates from leguminous trees growing in Africa and Latin America

Article Abstract:

Rhizobial isolates from Acacia senegal, Prosopis chilensis and related leguminous trees growing in Africa and Latin America were investigated to study the diversity and phylogeny of nodA and nifH genes. Gene sequence analysis revealed that all the strains had similar host ranges and belonged to the genera Sinorhizobium and Mesorhizobium. Similar symbiotic gene types were found in different chromosomal backgrounds suggesting that transfer of symbiotic genes has occurred across species boundaries.

Plant genetics, Phylogeny (Botany), Plant phylogeny, Trees, Trees (Plants), Acacia, Acacia (Plant), Mesquite

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Diversity and depth-specific distribution of SAR11 cluster rRNA genes from marine planktonic bacteria

Article Abstract:

Two subgroup of the alpha subclass of Proteobacteria, containing the small-subunit ribosomal DNA SAR11 cluster, have a depth-specific distribution in the midoceanic and coastal waters of the Pacific and North Atlantic Oceans. Two genes of the SAR11 cluster from the marine planktonic bacteria are PCR chimeras. Independent recovery from gene libraries determined the biological origin of the other bacteria. The genetic diversity in the SAR11 gene cluster represents macroevolutionary divergence.

Pacific Ocean, Natural history, Marine bacteria, North Atlantic Ocean

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Subspecies-dependent regulation of Bacillus thuringiensis protoxin genes

Article Abstract:

Plasmids containing fusions of the cry1 regulatory regions to lacZ were introduced into a variety of Bacillus thuringiensis subspecies to analyze the regulation of expression of the individual cry1 genes in greater detail. Findings showed subspecies-dependent differences in expression, as confirmed by measuring protoxin antigen and mRNA contents. Regions upstream of the promoters which are unique to each cry gene were found to be essential for this regulation.

Bacillus thuringiensis, Genetic regulation

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