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Biological sciences

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Construction and characterization of an Escherichia coli strain genetically engineered for Ni(II) bioaccumulation

Article Abstract:

Researchers created a strain of E. coli that can accumulate nickel by introducing the nixA gene from Helicobacter pylori. This gene codes for a nickel transport system. The resulting strain accumulated four times as much nickel as the native strain.

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Genetici engineering of Escherichia coli for enhanced uptake and bioaccumulation of mercury

Article Abstract:

Genetically engineered bacteria with synthetic phytochelatins (ECs) on their cell surface could be used to clean up toxic metals in the environment. Phytochelatins are naturally occurring metal-binding peptides and the synthetic version has the repetitive metal-binding motif (Glu-Cys)(sub n)Gly.

Environmental aspects, Chelating agents, Mercury, Mercury (Metal)

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Use of a modified Bacteroides-Prevotella shuttle vector to transfer a reconstructed beta-1,4-D-endoglucanase gene into Bacteroides uniformis and Prevotella ruminicola B(sub 1)4

Article Abstract:

The reconstruction of a carboxymethyl cellulase (CMCase) gene from the ruminal bacteria Prevotella ruminicola B(sub 1)4 allows the transfer of the CMCase gene from Escherichia coli to Bacteroides uniformis 0061 and then to other P. ruminicola B(sub 1)4 cells. The reconstruction involves the addition of a cellulose binding region from a Thermomonospora fusca cellulase. The gene is transferred using a chloramphenicol and tetracycline resistance shuttle vector. B. uniformis produces CMCase in the presence of a xylanase promoter and grows if two exocellulases are added to the culture medium.

Observations, Escherichia coli, Bacteriology, Rumen, Rumen microbiology

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