A fluorescent Gram stain for flow cytometry and epifluorescence microscopy

Article Abstract:

A Gram staining technique utilizing fluorescent nucleic acid binding dyes, hexidium iodide (HI) and SYTO 13, was developed for analyzing unfixed organisms in suspension by means of flow cytometry and epifluorescence microscopy. Microscopic analysis found gram-negative strains were stained red-orange by the HI, while gram-positive strains were dyed bright green by the SYTO 13. These results were confirmed by flow cytometric analysis. Quantitative assessments were made in terms of intensity and percentage of staining.

Methods, Flow cytometry, Stains and staining (Microscopy), Stains (Microscopy), Fluorescence microscopy

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Rapid microbial production of filamentous sulfur mats at hydrothermal vents

Article Abstract:

Rapid microbial formation of filamentous sulfur mats at Pacific hydrothermal vents by a chemoautotrophic, hydrogen sulfide-oxidizing bacterium has been documented in both shipboard and in situ experiments. It was found that formation of the sulfur mats may play a factor in the initial colonization of hydrothermal surfaces by macrofaunal Alvinella worms. This metabolic capability may be a significant source of microbial organic matter formation at deep-sea hydrothermal vents.

Research, Microbial mats, Marine bacteria, Hydrothermal vents, Sulfur

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