Mu DNA reintegration upon excision: evidence for a possible involvement of nucleoid folding

Article Abstract:

The position of the original insertion site of the mutant Mugem2ts prophage determines the position of the reintegration site. In this phage, the excised phage DNA is not lost but is reintegrated at another point on the same DNA molecule.

Bacteriophages, Transposons

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A two-hybrid system based on chimeric operator recognition for studying protein homo/heterodimerization in Escherichia coli

Article Abstract:

A two-hybrid system has been developed for studying protein homo/heterodimerization in Escherichia coli. It is based on chimeric operator recognition and seems to be a promising alternative to the various two-hybrid methods used to study protein-protein interactions. A lambdoid chimeric operator is recognized by a hybrid repressor formed by two chimeric monomers, the C-terminal domains of which are made up of heterologous proteins. Only if the proteins dimerize efficiently in vivo is a functional repressor formed and able to bind the chimeric operator and shut off the synthesis of a downstream reporter gene.

Italy, Statistical Data Included, Methods, Usage, Physiological aspects, Genomes, Mosaicism, Cell division, Protein research, Bacteriophage lambda

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Two-hybrid assay: construction of an Escherichia coli system to quantify homodimerization ability in vivo

Article Abstract:

A study was conducted to analyze the construction of an Escherichia coli system to quantify homodimerization ability in vivo. Two techniques were utilized to test protein dimerization. Results indicated that the construct has the potential to be integrated in any target gene oft he bacterial host, allowing the hybrid assay to be carried out in every bacterial genus where the reporter gene can be expressed.

Bacteria

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