An RNA 5'-Triphosphatase to the protein tyrosine phosphatases

Article Abstract:

RNA triphosphatase, guanylyl-transferase and methyltransferase are enzymatic activities wherein sequential action is needed by mRNA capping. The gene (CEL-1) is believed to encode the C.elegans capping enzymes and has a C-terminal domain having motifs detected in yeast and vaccinia virus capping enzyme guanylyltransferases. The N-terminal domain of CEL-1 has a noticeable sequence resemblance to the protein tyrosine phosphatase (PTP) enzyme family although it does not have detectable PTP activity. Furthermore, this domain is not similar to vaccinia virus capping enzyme even though it has RNA triphosphatase activity.

Research, Proteins, Messenger RNA

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Use of dsRNA-mediated genetic interference to demonstrate that frizzled and frizzled 2 act in the wingless pathway

Article Abstract:

Double-stranded RNA (dsRNA) was infused into Drosophila embryos to interfere with the gene function and determine the process of signal transduction of Drosophila Wnt-1 ortholog Wingless (Wg). The injection demonstrated that dsRNA effectively and specifically constrains the activities of wg, fushi tarazu, even-skipped and tramtrack. By comparison, single-strand RNA did not have any potency. When combined, interference of the frizzled and Drosophila frizzled 2 genes led to embryonic patterning defects that emulate loss of wingless function. However, single interference did not alter patterning.

Genetic engineering, Cellular signal transduction, RNA, Drosophila

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