Effect of sample holding time on recovery of Cryptosporidium oocysts and Giardia cysts from water samples

Article Abstract:

Processing times of up to two weeks may be acceptable for testing water samples for Cryptosporidium and Giardia. The US EPA requires samples to be processed much more quickly, but many laboratories cannot meet these strict requirements.

Testing

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Occurrence of cryptosporidium oocysts and giardia cysts in sewage in Norway

Article Abstract:

Samples of sewage influent from 40 sewage treatment works (STW) throughout Norway were screened for Cryptosporidium oocysts and Giardia duodenalis cysts. Data resulting from the study suggest that giardiasis is more widespread, and occurs with greater intensity of infection as compared to cryptosporidiosis in Norway as many STW have minimal treatment and discharge effluent into rivers and lakes, thus, risking contamination

Science & research, Norway, Research, Analysis, Physiological aspects, Sewage, Sewage microbiology, Polymerase chain reaction, Electrophoresis

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An evaluation of methods for the simultaneous detection of Cryptosporidium oocysts and Giardia cysts from water

Article Abstract:

An evaluation of the techniques for the detection of Cryptosporidium oocysts and Giardia cysts from tap and river water indicates that the calcium carbonate flocculation method gives the best results. Increasing the centrifugation to 5,000 x g increases the effectiveness of the recovery process for cysts and oocysts. Concentrations of oocysts improve with extra washes in the cartridge filtration method. Cellulose acetate membranes with a pore size of 1.2 micrometer and cellulose nitrate membranes with a pore size of 3.0 micrometer are effective for Cryptosporidium oocysts and Giardia cysts, respectively.

Aquatic microbiology

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