Analysis of Tox5 gene expression in Gibberella pulicaris strains with different trichothecene production phenotypes

Article Abstract:

The sequence analysis of the Tox5 promoter regions from geographically significant strains of Gibberalla pulicaris demonstrate the existence of Tox5-1 and Tox5-2. Strains producing high levels of trichothecenes carry a 42-nucleotide (nt) repeat sequence (Tox5-1), while those with low levels carry a single copy of the sequence (Tox5-2). Genetically crossing high and low-level trichothecene producers gives a cosegreggation of higher-level trichothecene production with Tox5-1.

Analysis

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Isolation and characterization of Tri3, a gene encoding 15-O-acetyltransferase from Fusarium sporotrichioides

Article Abstract:

The acetyltransferase gene (Tri3) of Fusarium sporotrichioides is related to the three genes of the trichothecene biosynthetic pathway. Tri3 has four introns. The Tri3 open reading frame encodes a protein with a molecular weight of 57,418 Da. Tri3 encodes the 15-O-acetyltransferase, which converts 15-decalonectrin to calonectrin. The disruption of the gene produces an excess of the deacetylated calonectrins and the blockage of 15-O-acetylation.

Observations

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The TRI11 gene of Fusarium sporotrichioides encodes a cytochrome P-450 monooxygenase required for C-15 hydroxylation in trichothecene biosynthesis

Article Abstract:

Research was performed to determine the characterization of TRI11 gene from the trichothecene gene cluster in Fusarium sporotrichioides. The nucleotide sequence of TRI11 predicts a polypeptide of 492 residues with considerable likeness to members of the cytochrome P-450 superfamily. Evidence suggests that disruption of TRI11 leads to an altered trichothecene production phenotype characterized by the accumulation of isotrichodermin.

Genetic aspects, Fungi

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