The role of the cyclic nucleotide phosphodiesterase of Dictyostelium discoideum during growth, aggregation, and morphogenesis: overexpression and localization studies with the separate promoters of the pde

Article Abstract:

A study was conducted on the effects of overexpression in of cyclic nucleotide phosphodiesterase (PDE) from constructs made from a fusion of the pde coding sequence and promoter-containing regions on Dictyostelium discoideum development. PDE overexpression from promoters specific to vegetative growth and aggregation resulted in an altered timing of aggregation, although a normal final morphology was obtained. PDE overexpression from promoters specific to late development (prL) resulted in an aggregation similar to that of control transformants, but without fruiting body formation. Cells expressing prL-lacZ were found to be distributed homogenously and were incorporated into the stalk during culmination.

Genetic aspects, Cell aggregation, Phosphodiesterases

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Regulation of Dictyostelium early development genes in signal transduction mutants

Article Abstract:

The early genes phosphodiesterase (PDE) and its glycoprotein inhibitor (PDI), of Dictyostelium amoebae undergo a two-stage regulation by a G-alpha2/G-beta-independent mechanism. PDI is sensitive to the protein kinase A catalytic subunit (PKAcat) which is essential for preparing the PDI transcript. Several mutants having errors in signal transduction are used to detect the dependence of the regulation of the PDE and the PDI transcripts on the receptors, PKA cat, G-alpha2, G-beta and adenylyl cyclase.

Genetic regulation, Genetic transcription, Transcription (Genetics), Transduction, Transduction (Genetics)

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The phosphodiesterase secreted by prestalk cells is necessary for Dictyostelium morphogenesis

Article Abstract:

Experimental studies on Dictyostelium discoideum reveal that a cyclic nucleotide phosphodiesterase (PDE) secreted by prestalk cells is essential for the morphogenesis of Dictyostelium. Homologous recombination helps destroy the inhibitor gene and development is not affected by the loss of the gene. Inhibition of PDE leads to an elongated tip, inhibition of slug formation and failure of culmination.

Observations, Cell research, Cytological research

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