Diversity and seasonal fluctuations of the dominant members of the bacterial soil community in a wheat field as determined by cultivation and molecular methods

Article Abstract:

Researchers in the Netherlands have found that the composition of soil bacteria wheat fields in July is very different from the composition in other months. The diversity of soil bacteria must be determined because current and future agricultural practices could have a substantial impact on them.

Analysis, Soil microbiology, Microbial populations

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Effect of genetically modified Pseudomonas putida WCS358r on the fungal rhizosphere microflora of field-grown wheat

Article Abstract:

Pseudomonas putida that has been genetically modified to produce the antifungal compound phenazine-1-carboxylic acid can suppress fungal growth in soil rhizospheres. The Antifungal compound is produced by the phz gene from Pseudomonas fluorescens 2-79.

Microbial genetic engineering, Soil fungi

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Analysis of fungal diversity in the wheat rhizosphere by sequencing of cloned PCR-amplified genes encoding 18S rRNA and temperature gradient gel electrophoresis

Article Abstract:

Fungal diversity in the wheat rhizosphere was analyzed using polymerase chain reaction (PCR) primers for the specific amplification of fungal 18S rDNA and temperature gradient gel electrophoresis. DNA was extracted from the wheat rhizosphere, and 18S rDNA gene banks were constructed in Escherichia coli by cloning PCR products generated with primer pairs. The primer pair EF4-EF3 appeared slightly biased to amplify Basidiomycota and Zygomycota, whereas EF4-fung5 amplified mainly Ascomycota.

Agriculture, Usage, Genetic aspects, Fungi, Gel electrophoresis, Wheat, Fungi in agriculture

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