Application of fibre-FISH (fluorescence in situ hybridization) to filamentous fungi: visualization of the rRNA gene cluster of the ascomycete Cochliobolus heterostrophus

Article Abstract:

Fibre fluorescence in situ hybridization (fibre-FISH) has been applied to an ascomycete. The goal was visualization of the Cochliobolus heterostrophus rRNA gene cluster. C. heterostrophus was found to have 90+ copies of the repeat unit in its rDNA. The stretching degree was similar to the value based on the Watson-Crick model. Fibre-FISH is not known to have been used in filamentous fungi.

Fungi, In situ hybridization

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A chromosome map of the European stone fruit yellows phytoplasma

Article Abstract:

A physical chromosome map has been made of the European stone fruit yellows (ESFY) phytoplasma strain GSFY1 as a first step toward a detailed genetic characterization of the ESFY phytoplasma. ESFY phytoplasma is an important prokaryotic pathogen that infects most kinds of stone fruits in Europe. The map, made using PFGE-purified genomic DNA from diseased tobacco and tomato plants, was not identical to that of the closely related apple proliferation phytoplasma, but was similar.

Germany, Identification and classification, Gel electrophoresis, Plant-pathogen relationships, Ribosomes, Circular DNA, Stone fruit, Stone fruits

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Cyclic heptapeptide microcystin biosynthesis requires the glutamate racemase gene

Article Abstract:

Cyclic heptapeptide microcystin biosynthesis has been found to require the glutamate racemase gene in the first reported research of the racemase in prokaryotic nonribosomal peptide synthetase (NRPS). The role of mcyF in microcystin synthesis has been studied using gene-disruption and complementation analyses.

Physiological aspects, Cyanobacteria, Peptides, Cladistic analysis, Ligases

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