Beta-tubulin mRNA as a marker of Cryptosporidium parvum oocyst viability

Article Abstract:

The viability of waterborne Cryptosporidium parvum oocysts can be assessed using beta-tubulin mRNA as marker. To prove this observation, rRNA and mRNA were investigated in a reverse transcription-PCR assay as potential markers of oocyst viability. mRNA levels decreased gradually after prolonged incubation of initially viable oocysts at room temperature, which coincided with the loss of oocyst infectivity to neonatal mice under study. The postmortem decay of two mRNA species shows that reverse transcription-PCR analysis can elucidate the viability of Cryptosporidium parvum oocysts.

Microorganisms, Genetic markers, Tubulins, Tubulin, Messenger RNA

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Sequence polymorphism in the beta-tubulin gene reveals heterogeneous and variable population structures in Cryptosporidium parvum

Article Abstract:

Research was conducted to examine sequence polymorphism in the 84-bp intron within the beta-tubulin gene of Cryptosporidium parvum. The limited resolution of the restriction fragment length polymorphism analysis necessitated the use of the sequence. Results provide strong evidence that untranslated regions are suitable targets for the application of beta-tubulin markers for genotyping C. parvum isolates.

Introns

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Extensive polymorphism in Cryptosporidium parvum identified by multilocus microsatellite analysis

Article Abstract:

Several microsatellite loci of Cryptosporidium parvum display substantial polymorphism. Some loci have as many as seven alleles, with differences between alleles ranging from one to 27 bp.

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