Carbohydrate and amino acid fermentation in the free-living primitive protozoan Hexamita sp

Article Abstract:

Research was performed to determine the primary products of glucose and amino acid fermentation of Hexamita sp. and to assess the influence of different O2 tensions on the fermentative metabolism of the flagellate. Results revealed that production of organic acids is more substantial under microaerobic conditions and decreased considerably under anaerobic and aerobic conditions. Further evidence suggests that Hexamita cells contain the arginine dihydrolase pathway, the catabolism rate of which is higher under anoxic conditions than microaerobic conditions.

Environmental aspects, Bacteria, Bacteria, Aerobic, Aerobic bacteria, Anaerobic bacteria, Cell motility, Protozoa, Protozoans

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Hydrogenosomes of Metopus contortus physiologically resemble mitochondria

Article Abstract:

The presence of calcium in the hydrogenosomes of Metopus contortus was analyzed via confocal laser scanning microscopy (CLSM) to determine the physiological characteristics of the organelle. CLSM and energy-dispersive X-ray microanalysis of Metopus contortus hydrogenosomes indicated the presence of calcium which was detected by the Fluo 3-AM calcium probe. The Metopus contortus organelle was also composed of hydrogenosomal inclusions or electron-dense granules that contained high levels of magnesium and phosphate.

Microbiological chemistry, Cell organelles, Organelles, Cell physiology, Ciliata, Ciliates

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Flow cytometry and other techniques show that Staphylococcus aureus undergoes significant physiological changes in the early stages of surface-attached culture

Article Abstract:

A study was conducted to analyze the physiology of Staphylococcus aureus in the early stages of surface-attached culture. Flow cytometry, scanning and transmission electron microscopy were utilized to carry out the analysis. Results indicated that the size and protein content of attached bacteria different with the growth phase. Findings also showed that attached and planktonic bacteria showed thicker cell walls in stationary phase than in exponential phase.

Usage, Cells (Biology), Staphylococcus aureus, Flow cytometry, Cells, Transmission electron microscopes, Scanning electron microscopes

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