Cell surface properties of organic solvent-tolerant mutants of Escherichia coli K-12

Article Abstract:

Microbiological research shows that genetically-modified bacteria with raised tolerance to organic solvents have an increased tolerance to water. Cell surface studies using mutants of the bacteria Escherichia coli K-12 demonstrate reduced hydrophobicity caused by raised lipopolysaccharide content. Variations to the organic solvent-tolerant microorganisms's lipopolysaccharide, OmpF and peptidoglycan-binding lipoprotein are thought to be responsible for increased organic solvent tolerance.

Cell membranes, Plasma membranes, Toxicology

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Purification of extracellular cholesterol oxidase with high activity in the presence of organic solvents from Pseudomonas sp. strain ST-200

Article Abstract:

The extracellular cholesterol oxidase of Pseudomonas sp. strain ST-200 was purified to investigate the role of this activity in ST-200 strain-mediated cholesterol oxidation in an organic solvent. The oxidase effectively oxidized cholesterol in the organic solvents but was not as effective in oxidizing cholesterol in the assay solution. It is speculated that oxidase had a mediating effect in the early stages of the cholesterol conversion reactions in the ST-200 culture.

Bacteria, Pseudomonas, Oxidases, Cholesterol

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Appearance of a stress-response protein, phage-shock protein A, in Escherichia coli exposed to hydrophobic organic solvents

Article Abstract:

A 28 kDa Escherichia coli protein associated with the inner membrane was induced strongly in K-12 cells grown in the presence of a hydrophobic organic solvent. The solvent suppressed the growth of E. coli. A partial amino acid sequence revealed that this protein was the phage-shock protein PspA. Findings indicated that E. coli cells are subject to strong stress in the presence of organic solvents.

Escherichia coli, Bacterial proteins

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