Cloned microsatellite repeats differ between 4-base restriction endonucleases

Article Abstract:

Simple sequence repeats (SSR), or microsatellite loci, have been use for parentage analysis, genetic mapping and studies of population structure but the development of novel loci is difficult and time consuming. A new study attempts to clone di-, tri-, and tetra-nucleotide repeat microsatellites for use in studying the population genetics of two wild plant species, Heliconia acuminata and Corythophora alta. The study shows that the numbers and repeat types of possible SSR markers are dependent upon the restriction endonuclease used to sample the genome during construction of DNA libraries. Details of the study are presented.

Genetic aspects, Plant genetics, Hybridization, Restriction enzymes, DNA, DNA restriction enzymes

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Mitochondrial DNA variability in Grauer's Gorillas of Kahuzi-Biega National Park

Article Abstract:

Eastern lowland gorillas (Gorilla gorilla graueri) are not well studied, particularly on a molecular level. The internal region of the mitochondrial DNA cytochrome oxidase subunit II (COII) areas and a hypervariable section of the mitochondrial DNA control region (D-loop) from wild lowland gorillas in montane and lowland habitats of Kahuzi-Biega National Park, were sequenced. The COII regions were found to be identical, suggesting previous diagnostic sites may be valid. There was low variability in the D-loop and haplotype frequencies were different in the two habits, indicating demographic distinctions.

Physiological aspects, DNA, Habitats, Mitochondrial DNA, Animals, Cytochrome oxidase, Gorillas

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