Cloning and partial characterization of two chromosomal loci from Bacteroides ovatus that contain genes essential for growth on guar gum

Article Abstract:

Transposon-generated mutants (M-4, M-5 and M-7) of Bacteroides ovatus unable to utilize guar gum were analyzed. Restriction analysis showed M-4 and M-5 contained the transposon at the same site, while M-5 and the M-7 loci did not overlap. The wild type clone of the M-5 region failed to form transconjugants in complementation experiments, while the M-7 clone failed to complement M-7 mutants. Two promoter regions were identified in the M-7 locus. This locus appears to have a regulatory function, since multiple copies of an M-7 fragment containing one promoter affected proteins not encoded on the segment.

Genetic aspects, Enzymes, Carbohydrate metabolism, Anaerobic bacteria, Enzyme regulation

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Regulation and cloning of the gene encoding amylase activity of the ruminal bacterium Streptococcus bovis

Article Abstract:

The regulatory phenomena in the amylase production by Streptococcus bovis and the molecular characteristics of this activity are discussed. Nutritional and time-course expriments were used in the examination of the amylolytic activity and the cloning and expression of the gene in Eschirichia coli and Bacillus subtilis. The utility of the amylase gene for the identification of S. bovis strains as agenetic probe was demonstrated.

Research, Analysis, Usage, Streptococcus, Cloning, Amylases

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