Cloning, expression and nucleotide sequence of lipase gene from Pseudomonas fluorescens B52

Article Abstract:

A lipase gene from Pseudomonas flourescens B52 was cloned and sequenced by the dideoxy nucleotide chain termination method. P. fluorescens is a psychotrophic bacterium causing dairy spoilage. Sequence analysis revealed an open reading frame of 1,428 nucleotides encoding a proteinof 476 amino acids with a molecular weight of 50,241. Analysis of the amino acid sequence showed the presence of a putative substrate-binding site common to all previously sequenced lipases. However, the rest of the residues showed few similarities to those of other Pseudomonas species.

Analysis, Pseudomonas fluorescens, Lipase

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Effect of phosphate limitation on synthesis of periplasmic cyclic beta-(1,2)-glucans

Article Abstract:

Analysis of the influence of phosphate limitation on the synthesis of periplasmic cyclic beta-(1,2)-glucans by Rhizobium meliloti and Agrobacterium tumefaciens reveals that phosphate limitation leads to significant reduction in the level of phosphoglycerol substituents in the periplasmic cyclic beta-(1,2)-glucans. R. meliloti produces anionic cyclic beta-(1,2)-glucans having high levels of succinic acid substituents. Neutral cyclic beta-(1,2)-glucans are produced by A. tumefaciens C58.

Physiological aspects, Phosphates, Gram-negative bacteria, Microbiological synthesis

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Synthesis of a low-molecular-weight form of exopolysaccharide by Bradyrhizobium japonicum USDA 110

Article Abstract:

Bradyrhizobium japonicum produces a low-molecular-weight form of exopolysaccharide that consists of a dimer of the pentasaccharide repeating unit. These carbohydrates may be involved in osmotic adaptation.

Microbial polysaccharides

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