Molecular cloning, structural analysis, and expression in Escherichia coli of a chitinase gene from Enterobacter agglomerans

Article Abstract:

The amino acid sequence of the chitinase gene, chiA, from the soilborne Enterobacter agglomerans was analyzed after its cloning and expression in Escherichia coli. The chitinase gene which codes for endochitinase and designated as Chia_Entag exhibited nucleotide and polypeptide sequences that are similar to that of Chia_Serma from Serratia marcescens. Furthermore, the solvent-accessible sites of the cloned Chia_Entag exhibited amino acid substitutions and yielded an open reading frame that codes for 562 amino acids of a 61-kDa precursor protein.

Analysis, Nucleotide sequence, Base sequence, Microbial enzymes

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Pantoea agglomerans strain EH318 produces two antibiotics that inhibit Erwinia amylovora in vitro

Article Abstract:

The presence of pantocin A and pantocin B, two distinct antibiotics, were detected in research on the EH318 strain of Pantoea agglomerans, a member of the Enterobacteriaceae. Research involved restriction maps, spectrum-of-activity data and cross-hybridization results using P. agglomerans marker exchange mutants and subclones; both antibiotics act against the fire blight causal agent Erwinia amylovora.

United States, Statistical Data Included, Antibiotics, Bacterial genetics, Agricultural bacteriology, Bacteriology, Agricultural

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Distribution and replication of the pathogenicity plasmid pPATH in diverse populations of the gall-forming bacterium Pantoea agglomerans

Article Abstract:

The evolution of pPATH plasmids is traced. These plasmids are found to have evolved from a common ancestral mobilizable plasmid that transformed into different strains of Pantoea agglomerans.

Science & research, Technology application, Plasmids, Enterobacter, Restriction fragment length polymorphism analysis

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