Combined expression of Aspergillus nidulans endoxylanase X24 and Aspergillus oryzae alpha-amylase in industrial baker's yeasts and their use in bread making

Article Abstract:

Expression of the genes coding for Aspergillus nidulans endoxylanase X24 and Aspergillus oryzae alpha-amylase in industrial baker's yeasts was investigated by molecular methods. The results revealed that expression of these enzymes in yeasts which are used for bread making influences the quality of bread. Yeast transformation experiments showed that bread made with transformants expressing both enzymes exhibits increased volume and reduced firmness.

Research, Usage, Bread, Enzymes, Yeast, Yeast (Food product), Enzyme synthesis

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Production of recombinant [beta]-hexosaminidase A, a potential enzyme for replacement therapy for Tay-Sachs and Sandhoff diseases, in the methylotrophic yeast Ogataea minuta

Article Abstract:

Recombinant [beta]-hexosaminidase A (HexA) was produced in the methylotrophic yeast Ogataea minuta to prepare a large amount of HexA for treatment based enzyme replacement therapy (ERT). The problem of antigenicity due to differences in N-glycan structures between mammalian and yeast glycoproteins was resolved by using [alpha]-1,6-mannosyltransferase deficient yeast as the host.

Physiological aspects, Genetic aspects, Mammals, Yeast fungi, Yeasts (Fungi)

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