Comparison of the BAX for screening/E. coli O157:H7 method with conventional methods for detection of extremely low levels of Escherichia coli O157:H7 in ground beef

Article Abstract:

The performance of the BAX for Screening/Escherichia coli O157:H7 was compared with the performance of the BAX system assay with traditional culture, an immunodiffusion method and enhanced selective agars. Focus was made on the detection of E. coli O157:H7 from ground beef samples injected with fewer than 3 CFU/g of organisms. Results suggest that the BAX for Screening/E. coli O157:H7 assay performed better than the other conventional methods by showing a detection rate of 96.5% compared to only 71.5% and 39% for the immunodiffusion and the best cultural methods, respectively.

Escherichia coli, Bacteria, Pathogenic, Pathogenic bacteria, Microbiological assay

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Epidemiological typing of Bacillus spp. isolated from food

Article Abstract:

A comparison of biotypes, fatty acid profiles and restriction fragment length polymorphisms of a PCR product identified 51 Bacillus isolates in milk samples and 11 isolates in food samples. Isolates with mesophilic or psychotropic characteristics were distributed into specific fatty acid or PCR-RFLP groups. Fatty acid clusters found predominantly in milk samples suggest that certain dairy plants have plant-specific B. cereus, which are responsible for postpasteurization contamination.

Food, Food microbiology, Bacillus (Bacteria), Bacillus, Bacteriology

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Direct detection of Brucella spp. in raw milk by PCR and reverse hybridization with 16S-23S rRNA spacer probes

Article Abstract:

Polymerase chain reaction (PCR) assays are used in conjunction with a reverse hybridization method in the cloning and subcloning of the 16S-23S rRNA spacer regions of Brucella abortus, B. melitensis and B. suis in raw milk. Results indicate that all the Brucella strains tested have similar spacer sequences and very high interspecies relatedness. The data also suggest that PCR assays are very useful for detecting bacterial pathogens in raw milk.

Analysis, Polymerase chain reaction

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