Construction and characterization of two recombinant bacteria that grow on ortho- and para-substituted chlorobiphenyls

Article Abstract:

Two recombinant pathways that allow growth on ortho- and para-substituted chlorobiphenyls as a single source of carbon have been generated following the cloning and expression of the aromatic ring dehalogenation genes in biphenyl-growing, polychlorinated biphenyl-cometabolizing Comamonas testosteroni VP44. The variants were synthesized by transformation of strain VP44 with plasmids containing specific genes for dehalogenation of chlorobenzoates. Mineralization of para-chlorobiphenyls was confirmed by cell protein yield corresponding to complete oxidation of the two biphenyl rings.

Bacterial genetics, Anaerobic bacteria, Biphenyl compounds, Biphenyls (Class of compounds), Microbial genetics

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Cloning, expression, and nucleotide sequence of the Pseudomonas aeruginosa 142 ohb genes coding for oxygenolytic ortho dehalogenation of halobenzoates

Article Abstract:

A new class of oxygenolytic ortho-dehalogenation (ohb) genes has been cloned from 2-chlorobenzoate- and 2,4-dichlorobenzoate-degrading Pseudomonas aeruginosa 142. Two clones, DHFalphaF'(pOD22) and DHFalphaF'(pOD33), converted 2-chlorobenzoate to catechol and 2,4-dichlorobenzoate and 2,5-dichlorobenzoate to 4-chlorocatechol. The ohb DNA region is flanked by 14-bp imperfect inverted repeats at positions 56 to 69 and 5984 to 5997.

Nucleotide sequence, Base sequence, Cloning, Gene expression, Pseudomonas aeruginosa, Halogenation

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