Cooccurrence of ScDSP gene expression, cell death, and DNA fragmentation in a marine diatom, Skeletonema costatum

Article Abstract:

A novel death-specific gene, ScDSP, encoding a calcium-regulated protein is obtained from death-phase-specific subtraction cDNA library and gene expression profiles between the exponential, while death phases in a diatom Skeletonema costatum are compared using the subtraction hybridization technique and a quantitative reverse transcription-PCR assay (Q-RT-PCR). The results have shown that ScDSP plays a role in the molecular mechanisms of self-destructive autolysis in phytoplankton under stress.

Cell death, Molecular dynamics

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Cooccurrence of elevated urea levels and dinoflagellate blooms in temperate estuarine aquaculture ponds

Article Abstract:

High urea levels associated with dinoflagellate blooms in temperate estuarine aquaculture ponds for hybrid striped bass have been investigated. On 10 of 14 occasions the blooms were found with urea levels above 1.5 (mu)M nitrogen. At lower levels, no evidence of dinoflagellate blooms was seen in seven samples. Factors determining differential growth of phytoplankton species have long been of interest. Urea and other organic nitrogen compounds should be put into models of nitrogen flow.

Statistical Data Included, Physiological aspects, Environmental aspects, Nitrogen (Chemical element), Nitrogen, Molecular microbiology, Urea, Dinoflagellates, Phytoplankton

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Microbial diversity in Maras salterns, a hypersaline environment in the Peruvian Andes

Article Abstract:

Maras salterns are located 3,380 m above sea level in Peruvian Andes and consists of more than 3000 ponds which are not interconnected and act as crystallizers where salt precipitates as these ponds are fed by hypersaline spring water rich in sodium and chloride. The microbiota inhabiting these salterns is analyzed by fluorescence in situ hybridization (FISH), 16S rRNA gene clone library analysis and cultivation techniques.

Methods, In situ hybridization, Biological diversity, Biodiversity, Ribosomal RNA, Structure, Chemical properties

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