Determination of total protein content of bacterial cells by SYPRO staining and flow cytometry

Article Abstract:

An assay was designed to measure protein biomass of marine planktonic bacteria by flow cytometry. The procedure was calibrated by employing five species of Bacteria isolated from seawater samples and grown in culture at various temperatures. The intensity of SYPRO-protein fluorescence of these bacteria strongly correlated with their total protein content, measured by the bicinchoninic acid method to be in the range of 60 to 330 fg of protein/cell.

Analysis, Usage, Bacteria, Flow cytometry, Proteins, Plankton, Planktonic bacteria

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Flow sorting of marine bacterioplankton after fluorescence in situ hybridization

Article Abstract:

The objective of the study was to develop a protocol for flow sorting hybridized prokaryotes, and to explore the usefulness of this protocol for aquatic microbial ecology. Results demonstrate that a combination of catalyzed fluorescent reporter deposition (CARD-FISH) and flow sorting can be useful for studying the diversity and the activity and the genomes of different bacterial populations in aquatic habitats.

Science & research, Observations, Biological diversity, Biodiversity, Marine bacteria, Hybridization

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Comparison of cellular and biomass specific activities of dominant bacterioplankton groups in stratified waters of the Celtic Sea

Article Abstract:

A clade of alpha-proteobacteria related to Roseobacter spp., a Cytophaga-Flavobacterium cluster, and a cluster of gamma-proteobacteria produce most of the total bacterioplankton metabolic activity in the Celtic Sea. The smallest cells of the gamma-proteobacteria had the highest growth-specific rates, accounting for their dominance in nutrient-limited waters.

Microbial metabolism, Marine plankton

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