Distribution of Clostridium perfringens and fecal sterols in a benthic coastal marine environment influenced by the sewage outfall from McMurdo Station, Antarctica

Article Abstract:

Clostridium perfringens is one of the most successfully used indicators of fecal contamination in aquatic systems. In an effort to determine whether the disposal of domestic wastes affects the coastal marine environment, the distribution of C. perfringens in wastewater outfall of the McMurdo Station in the Antarctica was investigated. Results indicate the presence of the bacteria, along with the fecal sterol coprostanol, in sediment cores and intestinal contents of invertebrates and fishes in the area. However, distribution was limited with increased depth and distance from the outfall.

Research, Environmental aspects, Sewage, Clostridium infections, Antarctic regions, Marine pollution, Sterols, United States. McMurdo Station

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Comparison of fluorescence microscopy and solid-phase cytometry methods for counting bacteria in water

Article Abstract:

The traditional epifluorescent microscopy (EFM) techniques for counting fluorescently labeled bead and bacteria are compared to the solid-phase cytometry (SPC) method to establish the minimum number of cells needed per filter for reliable total-direct-count data. The results indicate that when the numbers of bacterial cells per filter are reduced below 10(super 5), the microscopic methods increasingly overestimated the true bacterial abundance.

Microbiological research

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Whole-genome shotgun optical mapping of Rhodospirillum rubrum

Article Abstract:

Three optical maps (Xbal, Nhel, and HindIII) of the Rhodospirillum rubrum strain ATCC 11170 genome that aided in sequence assembly are described. The utility of optical mapping's resolution range in microbial sequence assembly is shown, and comments on the advantages of optical mapping for solving additional problem in genomics are presented.

Optical Instrument and Lens Manufacturing, Optical instruments and lenses, Optical Microscopes & Parts, Usage, Optical microscopes

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