Congruent phylogenies of most common small-subunit rRNA and dissimilatory sulfite reductase gene sequences retrieved from estuarine sediments

Article Abstract:

PCR primers against dissimilatory sulfite reductase can be used to analyze the diversity of sulfate-reducing bacteria in sediments. This enzyme is only found in sulfate-reducing bacteria.

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Conservation of the genes for dissimilatory sulfite reductase from Desulfovibrio vulgaris and Archaeoglobus fulgidus allows their detection by PCR

Article Abstract:

Standard techniques help clone the genes for dissimilatory sulfite reductase from Desulfovibrio vulgaris. A new gene encoding a protein DsvD containing 78 amino acids is identified by nucleotide sequencing. The alpha- and beta-subunit sequences of dsvD gene exhibit significant homology with those of the dissimilatory sulfite reductase from Archaeoglobus fulgidus. DsvD plays a vital role in dissimilatory sulfite reduction and the high degree of sequence similarity helps construct conserved deoxyoligonucleotide primers to identify dissimilatory sulfide reductase.

Genetic aspects, Nucleic acid probes

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Distribution of sulfate-reducing bacteria in a stratified fjord (Mariager Fjord, Denmark) as evaluated by most-probable-number counts and denaturing gradient gel electrophoresis of PCR-amplified ribosomal DNA fragments

Article Abstract:

The concentration of sulfate reducing bacteria increases within and below the anaerobic and anoxic waters of the chemocline in the Mariager Fjord, Denmark. The most-probable-number (MPN) counts and denaturing gel electrophoresis of PCR-amplified 16S rRNA and DNA encoding rRNA give similar distribution values for bacteria. The total bacterial nucleic acid and mass are nearly the same in the whole water column of the fjord though the bacterial population is different. The bacteria isolated by MPN are related to the delta subdivision of sulfate-reducing proteobacteria.

Denmark, Observations, Natural history, Microbial populations, Fjords

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