Diurnal variation of cell proliferation in three bacterial taxa from coastal North Sea waters

Article Abstract:

The short-term variability of cell proliferation is studied in three bacterial populations from coastal North Sea waters at two seasons with contrasting day lengths, water temperatures and phytoplankton assemblages. It is suggested that the absence of visible DNA synthesis in marine bacterioplankton cells after DNA pulse-labeling must not be interpreted as an indication of cell 'inactivity'.

Genetic aspects, Cell proliferation, Planktonic bacteria, DNA synthesis

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Contribution of Archaea to total prokaryotic production in the deep Atlantic Ocean

Article Abstract:

The catalyzed reported deposition-fluorescence in situ hybridization (CARD-FISH) method combined with microautoradiography (MICRO-CARD-FISH) is used to determine the fraction of the bacteria, Crenarchaeota, and Euryarchaeota metabolically active in the deep waters of the North Atlantic. The results revealed a high fraction of metabolically active cells in the deep ocean.

Germany, Atlantic Ocean, Usage, Environmental aspects, Prokaryotes, Growth, Archaeabacteria, Company growth, Archaea

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Combining catalyzed reporter deposition-fluorescence in situ hybridization and microautoradiography to detect substrate utilization by bacteria and Archaea in the deep ocean

Article Abstract:

The newly formed CARD-FISH procedure is updated to identify the marine Archaea by interchanging the lysozyme permeabilization treatment with proteinase K. This method is used along with the microautoradiography to determine the Archaea, which outnumber the bacteria in the deep ocean of North Atlantic.

Models, Analysis, Proteases, Microbiology, Lysozyme, Marine archaeologists

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