Engineering of quasi-natural Pseudomonas putida strains for toluene metabolism through an ortho-cleavage degradation pathway

Article Abstract:

A bacterial catalyst for bioconversion of the aromatic hydrocarbon toluene and several alkyl and chloro- and nitro-substituted derivatives into the corresponding benzoates was developed by fully reassembling the upper TOL operon of plasmid pWWO of Pseudomonas putida as a single gene cassette along with its cognate regulatory gene, xylR. To match the corresponding DNA segment to the chromosome of a P. putida strain, a genetic method was used that facilitates the elimination of all recombinant tags carried over from earlier cloning steps and that leaves the natural strain bearing only the relevant DNA segment. The results are discussed.

Pseudomonas putida, Toluene, Aromatic compounds

---

phnE and glpT genes enhance utilization of organophosphates in Escherichia coli K-12

Article Abstract:

Organophosphoric compounds (OPCs) belong to a class of pesticides in use around the world. They account for a large number of poisonings in humans and pose hazards for the environment. Thus, microbial degradation of the compounds are of great interest for researchers. In an effort to better understand the metabolic process behind the microbial utilization of OPCs. spontaneous organophosphate utilization mutants of the Escherichia coli K-12 strain was investigated. Two genes responsible for the mutation were then identified using cloning experiments.

Escherichia coli, Organophosphorus compounds, Organic phosphorus compounds

---

Similar abstracts:

• Abstracts: Generation of a transient non-culturable state in Pseudomonas putida during detoxification of N-ethylmaleimide
• Abstracts: The origins of V(D)J recombination. Evolution of simple repeats in DNA and their relation to human disease
• Abstracts: Phylogenetic diversity of intra-amoebal legionellae as revealed by 16S rRNA gene sequence comparison. Molecular evidence for the existence of additional members of the order Chlamydiales
• Abstracts: Flowering gene Ppd in pea: map position and disturbed segregation of allele ppd-2. Stable association of a pigmentation allele with an oncogene: nonhybrid melanomas in Xiphophorus variatus
• Abstracts: Head spot and dilute mutations in the Norway rat. Parentage and kinship studies in an obligate brood parasitic bird, the brown-headed cowbird (Molothrus ater), using microsatellite DNA markers

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.