Phylogenetic diversity of intra-amoebal legionellae as revealed by 16S rRNA gene sequence comparison
Article Abstract:
The phylogenetic relationships of 10 'Legionella-like amoebal pathogen' isolates to the previously characterized Legionella lytica strains and other Legionella species were examined by determining their 16S rRNA gene sequences. All 10 isolates clustered within a monophyletic group which has all other members of the genus Legionella. Eight formed a monophyletic subgroup within the genus, which also included the two previously characterized L. lytica strains. Four shared a specific and very close relationship with the L. lytica type strain. The other two LLAP strains showed no specific evolutionary relationship to either L. lytica or any other Legionella species. LLAPs thus appear to be as evolutionarily diverse as the other Legionella species.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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Prototheca richardsi, a pathogen of anuran larvae, is related to a clade of protistan parasites near the animal-fungal divergence
Article Abstract:
Research was conducted to examine the enigmatic protist Protheca richardsi which has been implicated as a gut parasite of anuran larvae. Its cells were isolated from tadpole feces and DNA was purified by Qiagen chromatography. It was confirmed by in situ hybridization that the sequence was obtained from the parasite. Comparative sequence analysis revealed that P. richardsi is not an alga, but a member of the Ichthyosporea which is a newly recognized but previously untitled clade of protistan parasites near the animal-fungal divergence.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1999
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Molecular evidence for the existence of additional members of the order Chlamydiales
Article Abstract:
A study was conducted to determine the existence of additional members of the order Chlamydiales. A polymerase chain reaction was designed to determine all known Chalmydiaceae sequences in one reaction. Primers were chosen to amplify a fragment of the 16S rRNA gene. DNA was then isolated from peripheral blood mononuclear cells of health controls. Experimental results indicated a novel lineage separated from the genus Chlamydia with four known Chlamydial species.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1999
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