Ergosterol content in various fungal species and biocontaminated building materials

Article Abstract:

The ergosterol content of six filamentous fungi species, three yeast species and an actinomycete species has been measured in pure broth cultures. The ergosterol content of 40 building material samples contaminated with fungi and actinomycetes has also been determined. Gas chromatography and mass spectrometry were used in analyzing the samples which have been hydrolized in alkaline methanol. Results reveal the ergosterol content of the fungi species vary from 2.6 to 42 microg/ml of dry mas while values range from 0.017 to 86 microg/g of dry mass for building materials. There was no ergosterol found in the actinomycete culture.

Measurement, Sterols

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Heat shock response in the thermophilic enteric yeast Arxiozyma telluris

Article Abstract:

The heat shock response of two strains of the thermophilic yeast Arxiozyma telluris has been studied and has been compared with that of the mesophilic yeast Saccharomyces cerevisiae. A. telluris is found in the digestive tracts of warm-blooded domestic and wild animals and are obligate and facultative saprophytes. Mild heat shock was induced between 35 to 40 degrees C for 30 min for the proteins hsp 104, hsp 90 and hsp 60. Results indicate that the Arxiozyma strains have a heat shock response similar to S. cerevisiae even if the former have a narrow temperature range for growth.

Microbial metabolism, Heat shock proteins

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Use of solid-phase extraction to determine ergosterol concentrations in plant tissue colonized by fungi

Article Abstract:

Solid-phase extraction (SPE) is a good chromatographical method for analyzing ergosterol, by using sample extracts of plant tissue that contain colonies of filamentous fungi. Ergosterol is an important membrane lipid component of eumycotic fungi. The SPE technique has many advantages over the available liquid-liquid extraction methods. The lack of toxic substances makes the method safe for the user. The time required for analysis is reduced as the technique is simple.

Observations, Plant cells and tissues, Plant cells, Membrane lipids

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