In situ reverse transcription-PCR for monitoring gene expression in individual Methanosarcina mazei S-6 cells

Article Abstract:

Research has been conducted on the Methanosarcina mazei mRNA. The reverse transcription-PCR protocol to detect this mRNA is analyzed.

Cells (Biology), Genetic transcription, Transcription (Genetics), Cells, Messenger RNA

---

Expression of nifH genes in natural microbial assemblages in Lake George, New York, detected by reverse transcriptase PCR

Article Abstract:

Research has been conducted on the nitrogenase genes. The use of the reverse transcriptase polymerase chain reaction method in detecting the expression of these genes in a meso-oligotrophic lake is discussed.

---

Visualization of specific gene expression in individual Salmonella typhimurium cells by in situ PCR

Article Abstract:

The expression of the lac gene in Salmonella typhimurium was monitored using a modified polymerase chain reaction (PCR) technique. The lac messenger RNA levels was approximated using gene activity measures at the population level. This technique will provide researchers an opportunity to investigate specific gene expression in bacteria as well as determine bacterial spatial distribution in communities in natural habitats.

Usage, Genetic aspects, Bacterial genetics, Salmonella typhimurium

Similar abstracts:

• Abstracts: Elucidation of Listeria monocytogenes contamination routes in cold-smoked salmon processing plants detected by DNA-based typing methods
• Abstracts: Characterization of 16S rRNA genes from oil field microbial communities indicates the presence of a variety of sulfate-reducing, fermentative, and sulfide-oxidizing bacteria
• Abstracts: Cloning, expression, and sequence analysis of the gene encoding the alkali-stable, thermostable endoxylanase from alkalophilic, mesophilic Bacillus sp. strain NG-27
• Abstracts: Transformation of the antibacterial agent norfloxacin by environmental mycobacteria. Microbiological transformation of enrofloxacin by the fungus Mucor ramannianus
• Abstracts: Mena, a relative of VASP and Drosophila Enabled, is implicated in the control of microfilament. Putting on the brakes: a negative regulatory function for Ena/VASP proteins in cell migration

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.