Complementation cloning of NEMO, a component of the IkappaB kinase complex essential for NF-kappaB activation

Article Abstract:

The 5R mutant cell line was originally isolated as a cellular flat variant of Tax protein-transformed Rat-1 fibroblasts. The Tax protein is a transcription activator derived from the human T cell leukemia virus type 1 (HTLV-1). Characterization studies found 5R cells to be resistant to multiple NF-kappaB transcription factor activating stimuli, which made it ideal for use in the complementation cloning of the NF-kappaB Essential MOdulator (NEMO) gene. Results revealed that NF-kappaB activity is required for cell transformation.

Research, Cloning, Genetic transcription, Transcription (Genetics), Cytogenetics, Cell lines, Genetic transformation, HTLV-I (Virus)

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Yeast RNA polymerase II at 5 angstrom resolution

Article Abstract:

Single crystals of yeast RNA polymerase II without Tpb4 and Tpb7 have been grown for structural analysis and to test some ideas about the molecule. The result was structure determination to better than 6.0-angstrom resolution. Appropriate treatment os S-ray diffraction from an unoriented 18-heavy atom cluster derivative have significant phase information to 5-angstrom resolution. Some structure-function relationships which underlie the transcription mechanism have been suggested.

United States, Statistical Data Included, Analysis, Usage, Genetic aspects, RNA polymerases, Yeast, Yeast (Food product), X-ray diffractometer, X-ray diffractometers

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