Fungicides and sterol-deficient mutants of Ustilago maydis: plasma membrane physico-chemical characteristics do not explain growth inhibition
Article Abstract:
A study on the accumulation of abnormal sterols in the plasma membrane of the Ustilago maydis was conducted to analyze its effects. The areas of lipid composition and fluidity on lipid composition were also considered. The methods used in the research include subjecting U. maydis to a strained and cultured condition, plasma membrane purification, lipid analysis, passive diffusion constants for protons and others. Findings showed that the accumulation of abnormal sterols in plasma membranes do not result in fungicide-induced growth inhibition in U. maydis. Furthermore, no changes were observed in the fluidity of the plasma membrane.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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Detection of genetic variation in Ustilago maydis strains by probes derived from telomeric sequences
Article Abstract:
Genetic variation in basiodiomycetous fungus Ustilago maydis was investigated by fingerprint analysis with probes containing telomeric DNA. Group differences were observed between the endmost restriction fragments (EFs) and the endmost-associated restriction fragments (EAFs) of the chromosomes within strains. EFs showed similar lengths in two strains but were of different lengths in the other strains. Analysis of the EAFs showed the presence of distinct bands in the terminal and interstitial sites of the chromosomes in all strains.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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Analysis of fission yeast DNA structure checkpoints
Article Abstract:
The identification of DNA structure changes and the activation of downstream kinases Chk1 and Cds1 in 'Schizosaccharomyces pombe' and 'Saccharomyces cerevisiae' can be achieved through a core complex of six or more proteins. In this model, Cds1 is activated due to a response to DNA replication arrest or to DNA damage during S phase. On the other hand, Chk1 is phosphorylated in response to DNA damage but not to DNA arrest.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
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Agustin Hernandez
Fungicide-induced growth inhibition is a fact that is never questioned in this article. What this paper aimed at showing was that accumulation of abnormal sterols at the plasma membrane does not lead to growth inhibition through increased permeability or rigidity of this organelle. In other words, fungicide-induced growth inhibition's molecular causes must be looked elsewhere from the plasma mere membrane physical characteristics. In this sense, a putative cause is suggested: ATP depletion through plasma membrane proton ATPase hyperactivity.
Thank you, anyway, for considering my work
A. Hernandez