Growth from spores of nonproteolytic Clostridium botulinum in heat-treated vegetable juice

Article Abstract:

The addition of heat-treated vegetable juice to culture media can increase the number of heat-damaged spores of Clostridium botulinum that can lead to colony formation. This was found in a study that sought to measure the ability of spores of nonproteolytic C botulinum to survive heat treatment in vegetable juices and then lead to growth in the resulting vegetable juices. Tests of turnip, spring green, helda bean, broccoli or potato juice showed that unheated spores of nonproteolytic C botulinum were able to lead to growth, although the probability of growth was low and the time to growth longer than time to growth in culture media.

Physiological aspects, Bacteria, Pathogenic, Pathogenic bacteria, Heat, Vegetable juices

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Historical and contemporary NaCl concentrations affect the duration and distribution of lag times from individual spores of nonproteolytic Clostridium botulinum

Article Abstract:

The effect of NaCl concentration during sporulation and subsequent growth on the distributions of times associated with different stages of the lag phase of individual spores of nonproteolytic Clostridium botulinum strain Eklund 17B is determined. The results on distribution of lag times for individual spores have helped in understanding the mechanisms that can be used in improving the predictions of growth in food and refining risk assessments.

Salts, Chemical properties, Report

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Heterogeneity of times required for germination and outgrowth from single spores of nonproteolytic Clostridium botulinum

Article Abstract:

A novel attempt is made to determine the distributions of times associated with the various stages of germination and subsequent growth from spores and the relationships between these stages. As the variability in postgermination stages is relatively large, the first spore to germinate will not necessarily be the first spore to produce actively dividing cells and start neurotoxin production.

Genetic research

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