High-level production of human leptin by fed-batch cultivation of recombinant Escherichia coli and its purification

Article Abstract:

A study has been conducted to describe the development of an expression vector for leptin and optimization in induction as related to cell optical density during high-cell density cultivation of recombinant Escherichia coli for the production of gram quantities of recombinant human leptin. Leptin has been identified as a protein secreted by fat cells that influences body weight homeostasis. This study aims to exploit leptin as a regulator of a therapeutic agent to control human obesity.

Physiological aspects, Escherichia coli, Leptin, Recombinant proteins

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Engineering Escherichia coli for increased productivity of serine-rich proteins based on proteome profiling

Article Abstract:

Research discusses genetic approaches toward production enhancement of useful proteins in bacteia as exemplified by the overproduction of human leptin in Escherichia coli. The methodology involves proteome profiling of recombinant E. coli during the overproduction of leptin. The productivity of leptin is achieved by manipulating the cysK gene that has the potential to enhance the production of serine-rich proteins as well.

South Korea, Genetic aspects, Microbiological synthesis, Statistics, Statistics (Data), Bacterial proteins, Proteomics

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Rational design of a Corynebacterium glutamicum pantothenate production strain and its characterization by metabolic flux analysis and genome-wide transcriptional profiling

Article Abstract:

A 'second-generation' production strain was derived from a Corynebacterium glutamicum pantothenate producer by rational design to assess its potential to synthesize and accumulate the vitamin pantothenate by batch cultivation. The strategy successfully improved pantothenate production by genetically modified Corynebacterium glutamicum strains and revealed new constraints in attaining high productivity.

Pantothenic acid

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