Histidine phosphorylation of P-selection upon stimulation of human platelets: a novel pathway for activation-dependent signal transduction

Article Abstract:

Phosphorylation after platelet stimulation with thrombin or collagen produces phosphohistidine at histidines on the cytoplasmic tail of P-selectin.The two-component systems in prokaryotes which regulates significant physiological functions are marked by transient phosphorylation. But related events have not been involved in signal transduction in mammalian cells. To investigate histidine phosphorylation during stimulation of human cells, activated platelets were examined for the creation of protein phosphohistidine in a model system using P-selectin.

Blood platelets, Platelet activation

---

Cell cycle-dependent polar localization of an essential bacterial histidine kinase that controls DNA replication and cell division

Article Abstract:

A study was conducted to analyze CckA, an essential histidine kinase promoting CtrA activation by phosphorylation. The Beckton Dickinson FACStar Plus machine was utilized to prepare flow cytometry samples. Ultraviolet-mutagenized CB15N cells were plated on soft agar supporting a bacteriophage. Experimental results indicated that dynamic changes in cellular location of critical signal proteins promote a unique mechanism for the control of the prokaryote cell cycle.

Analysis, Prokaryotes, Cells (Biology), Cell cycle, Cells, Phosphorylation

---

Structure of CheA, a signal-transducing histidine kinase

Article Abstract:

A study was conducted to analyze the structure of CheA, a signal-transducing histidine kinase. Patterson map analysis was carried out to determine the mercury atoms positions of derivatized CheA. Experimental results indicated that CheA supports the nucleophilic histidine and activating glutamate for phosphotransfer. Findings also showed that relative domain motions connect receptor response to kinase activity.

Bacteria

Similar abstracts:

• Abstracts: Phosphorylation of the MAP kinase ERK2 promotes its homodimerization and nuclear translocation. Activation mechanism of the MAP kinase ERK2 by dual phosphorylation
• Abstracts: Termination of mammalian rDNA replication: polar arrest of replication fork movement by transcription termination factor TTF-I
• Abstracts: Multiple effects of mutation on expression of alternative cell surface protein genes in Tetrahymena thermophila
• Abstracts: Cloning and characterization of the abortive infection genetic determinant abiD isolated from pBF61 of Lactococcus lactis subsp. lactis KR5
• Abstracts: A perfect vulva every time: gradients and signaling cascades in C. elegans. Spatial and temporal controls target pal-1 blastomere-specification activity to a single blastomere lineage in C. elegans embryos

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.