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Identification of species and sources of Cryptosporidium oocysts in storm waters with a small-subunit rRNA-based diagnostic and genotyping tool

Article Abstract:

Researchers used a small-subunit rRNA-based PCR restriction fragment length polymorphism to identify 12 genotypes of Cryptosporidium in 29 water samples from a stream. This method can be used instead of an immunofluorescent assay.

Author: Lal, Altaf A., Xiao, Lihua, Limor, Josef, Alderisio, Kerri, Royer, Michael
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000

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Molecular characterization of Cryptosporidium oocysts in samples of raw surface water and wastewater

Article Abstract:

Researchers can use small-subunit rRNA-based PCR-restriction fragment length polymorphism to analyze water samples for the presence of Cryptosporidium. This technique can differentiate between Cryptosporidium strains that affect humans and those that do not.

Author: Xiao, Lihua, Limor, Josef, Lal, Altaf, Singh, Ajaib, Graczyk, Thaddeus K., Gradus, Steve
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001

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Genetic diversity of Cryptosporidium spp. in captive reptiles

Article Abstract:

The genetic diversity of cryptosporidium in reptiles was analyzed using PCR-restriction fragment length polymorphism and sequence analysis of a small subunit rRNA gene. In the study samples of snakes, lizards and tortoises were analyzed and results suggest extensive genetic diversity.

Author: Sulaiman, Irshad M., Lal, Altaf A., Xiao, Lihua, Limor, Josef, Graczyk, Thaddeus K., Arrowood, Michael J., Zhou, Ling, Ryan, Una M., Li, Lixia, Kombert, Mark, Koudela, Bretislav, Junge, Randy, Modry, David
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
Science & research, Research, Case studies, Biological diversity, Biodiversity, Reptiles

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Subjects list: Usage, Measurement, Water, Aquatic microbiology, Polymerase chain reaction, Coccidia, Cryptosporidium
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