Immunogold and fluorescein immunolabelling of Legionella pneumophila within an aquatic biofilm visualized by using episcopic differential interference contrast microscopy

Article Abstract:

Direct immunostaining procedures for detecting Legionella pneumophila growing in biofilms were described. The legionellae in the biofilms were labelled with a specific monoclonal antibody and with gold or fluorescein isothiocyanate conjugates. Episcopic differential interference contrast microscopy was used to view the stained biofilms. The immunogold staining procedure allowed rapid, clear and simultaneous visualization of unlabelled total biofilm flora and the gold-labelled legionellae.

Analysis, Legionella pneumophila

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Detection of methanotrophs in groundwater by PCR

Article Abstract:

Research was conducted to identify methanotrophic bacteria using polymerase chain reaction (PCR). The objective was to develop approaches for specifically detecting pmo in groundwater. Primers were designed based on regions that are different in pmoA and amoA which were responsible for specific amplification when DNA from a methanotroph was used as the template. Results confirmed the identity of the PCR through amplification or sequencing with a nested primer.

Research, Aquatic microbiology, Polymerase chain reaction, Water, Underground, Groundwater

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Localization of methanol dehydrogenase in two strains of methylotrophic bacteria detected by immunogold labeling

Article Abstract:

The localization of methanol dehydrogenase (MeDH) in a methylotrophic bacterium, Methylobacterium sp. strain AM1, and a marine methanotrophic bacterium, Methylomonas sp. strain A4, were detected by specific immunogold labelling, following MeDH antibody preparation. The AM1 and A4 MeDH were localized to the periplasm and to the intracytoplasmic membrane, respectively. The anti-A4 MeDH antibody demonstrated cross-reactivity in immunoblots.

Physiological aspects, Alcohol dehydrogenase, Alcohol dehydrogenases

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